Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146; 6x His-Tag or 10x His-Tag


Rabbit monoclonal to His-Tag (6xHis-Tag or 10xHis-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy)

SKU: 31-1048-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-His-Tag (6xHis-Tag or 10xHis-Tag) Rabbit Monoclonal Antibody [RM146]
Product DescriptionRabbit monoclonal to His-Tag. (6xHis-Tag or 10xHis-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy)
Catalog No.31-1048-00
Clone NameRM146
Product Data SheetAnti-His Tag Rabbit Monoclonal Antibody Data Sheet RM146
SpecificityRM146 reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
ImmunogenMixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
ApplicationWestern Blot
Flow Cytometry
Species ReactivityAll
Intended UseResearch Use Only
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.1 ug/mL – 1 ug/mL;
Immunoprecipitation: 0.5 ug/mL – 2 ug/mL;
Immunocytochemistry 0.5 ug/mL – 2 ug/mL;
Flow Cytometry: 0.5 ug/mL – 2 ug/mL;
Immunohistochemistry; 0.1 ug/mL-1 ug/mL.

Product Images

Western blot of 293T cells:
(1) untransfected or
(2) transfected with His-Tag fusion protein X and
(3) E. coli lysate without His-Tag Protein Y
(4) E. coli lysate with His-Tag Protein Y
Using RevMAb Clone RM146 at 0.2 ug/mL.


Immunoprecipitation of 293T cells expressing a
His-Tag fusion protein by RevMAb clone RM146,
was blotted with RM146. (1) Whole lysate control;
(2) IP by rabbit IgG control; (3) IP by RM146.


Immunohistochemistry staining of naïve HepG2 cells
(Negative control) using anti-His-Tag antibody, RM146.


Immunohistochemistry staining of 293T cells expressing
His-Tag nuclear protein X, using anti-His-Tag antibody,

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