Anti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody, Clone RM175; H3K27me3, Histone H3 (trimethyl K27)

$155.00$370.00

Rabbit monoclonal to Trimethylated Histone H3 Lysine 27, H3K27me3, Histone H3 (trimethyl K27)

Clear selection

Product Details
Product NameAnti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [RM175]
Product DescriptionRabbit monoclonal to Trimethylated Histone H3 Lysine 27, H3K27me3, Histone H3 (trimethyl K27)
Catalog No.31-1062-00
Clone NameRM175
Product Data SheetAnti-Trimethyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody Data Sheet RM175
SpecificityRM175 reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity non-modified Lysine 27, monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in histone H3.
ImmunogenA trimethyl-peptide corresponding to trimethyl-Histone H3 (Lys27).
ApplicationWestern Blot (WB)
Immunohistochemistry (IHC)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 1 ug/mL – 2 ug/mL;
IHC: 0.5 ug/mL – 2 ug/mL;
ELISA: 0.5ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
References for Trimethyl-Histone H3 (Lys27) antibody [RM175]Lu P et al. Genome encode analyses reveal the basis of convergent evolution of fleshy fruit ripening. Nat Plants. (2018) 10.1038/s41477-018-0249-z. ChIP; Plant. Read more (PubMed: 30250279)
.
Product Images
RM175

RM175 specifically reacts to Histone H3
trimethylated at Lysine 27 (K27me3). No
cross reactivity with non-modified Lysine 27
(K27 Ctrl), monomethylated Lysine 27
(K27me1) or dimethylated Lysine 27 (K27me2),
or other methylations in Histone H3.

Western

Western Blot of recombinant histone H3.3 (1) and
acid extracts of HeLa cells (2), using RM175 at
1 ug/mL, showed a band of histone H3 trimethyl-
ated at Lysine 27 (K27me3) in HeLa cells.

Immunohistochemistry

Immunohistochemistry staining of HepG2 cells using
Anti-Trimethyl-Histone H3 (Lys27) antibody, RM175.


Related Products

Size Format

25ug – $155, 100ug – $370

References

Lu P et al. Genome encode analyses reveal the basis of convergent evolution of fleshy fruit ripening. Nat Plants. (2018) 10.1038/s41477-018-0249-z. ChIP; Plant. Read more (PubMed: 30250279)