Anti-Trimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody, Clone RM157; H3K79me3, Histone H3 (trimethyl K79)

$155.00$360.00

Rabbit monoclonal to Trimethylated Histone H3 Lysine 79, H3K79me3, Histone H3 (trimethyl K79)

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Product Details
Product NameAnti-Trimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody [RM157]
Product DescriptionRabbit monoclonal to Trimethylated Histone H3 Lysine 79, H3K79me3, Histone H3 (trimethyl K79)
Catalog No.31-1053-00
Clone NameRM157
Product Data SheetAnti-Trimethyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody Data Sheet RM157

SpecificityRM157 reacts to Histone H3 trimethylated at Lysine 79 (K79me3). No cross reactivity with monomethylated Lysine 79 (K79me1), dimethylated Lysine 79 (K79me3), or other methylations in histone H3.
ImmunogenA trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys79).
ApplicationWestern Blot (WB)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.2 ug/mL – 1 ug/mL;
ELISA: 0.1 ug/mL - 0.5 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.

Keywords
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Product Images
A

A Peptide dotblot shows RM157 only reacts to Histone
H3 trimethyl-Lysine 79 (K79me3). No cross reactivity.
with nonmodified Lysine 79 (H3K79Ctrl), monomethylated.
Lysine 79 (K79me1), or dimethylated Lysine 79 (K79me2).

RM157

RM157 specifically reacts to Histone H3 trimethylated
at Lysine 79 (K79me3). No cross reactivity with other
methylated lysines in Histone H3.

Western

Western Blot of recombinant histone H3.3 (1)
and acid extracts of HeLa cells (2), using RM157
at 0.5 ug/mL, showed a band of histone H3
trimethylated at Lysine 79 (K79me3) in HeLa cells.


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