Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM135; H3K4me2, Histone H3 (dimethyl K4)

$155.00$370.00

Rabbit monoclonal to Dimethylated Histone H3 Lysine 4, H3K4me2, Histone H3 (dimethyl K4)

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Product Details
Product NameAnti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [RM135]
Product DescriptionRabbit monoclonal to Dimethylated Histone H3 Lysine 4, H3K4me2, Histone H3 (dimethyl K4)
Catalog No.31-1037-00
Clone NameRM135
Product Data SheetAnti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM135
SpecificityRM135 reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with monomethylated Lysine 4 (K4me1), trimethylated Lysine 4 (K4me3), or other methylations in histone H3.
ImmunogenA dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys4).
ApplicationWestern Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.02 ug/mL – 0.2 ug/mL;
ICC: 1 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.

Keywords
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Product Images
RM135

RM135 specifically reacts to Histone H3 dimethylated
at Lysine 4 (K4me2). No cross reactivity with mono-
methylated Lysine 4 (K4me1), trimethylated Lysine 4
(K4me3), or other methylations in histone H3.

Western

Western Blot of recombinant histone H3.3 (1) and
acid extracts of HeLa cells (2), using RM135 at 0.025
ug/mL, showed a band of histone H3 dimethylated
at Lysine 4 (K4me2) in HeLa cells.

Immunocytochemistry

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Dimethyl-Histone H3 (Lys4)
Rabbit mAb RM135 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).


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25ug – $155, 100ug – $370

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