Anti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody, Clone RM130; H3K14ac, Histone H3 (K14 acetylation)

$149.00$349.00

Rabbit monoclonal to Acetylated Histone H3 Lysine 14 (K14ac). H3K14ac, Histone H3 (K14 acetylation)

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Product Details
Product NameAnti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody [RM130]
Product DescriptionRabbit monoclonal to Acetylated Histone H3 Lysine 14 (K14ac), H3K14ac, Histone H3 (K14 acetylation)
Catalog No.31-1032-00
Clone NameRM130
Product Data SheetAnti-Acetyl-Histone H3 Lys14 Rabbit Monoclonal Antibody Data Sheet RM130
SpecificityRM130 reacts to Histone H3 acetylated at Lysine 14 (K14ac), and is not affected by the modification of neighboring amino acids. No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.
ImmunogenAn acetyl-peptide corresponding to Acetyl-Histone H3 (Lys14)
ApplicationWestern Blot (WB)
Immunocytochemistry (ICC)
Chromatin IP (ChIP)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL - 2 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ChIP: 2 ug/mL- 10 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
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Product Images
RM130

RM130 specifically reacts to Histone H3 acetylated
at Lysine 14 (K14ac). No cross reactivity with
acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine
18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac),
Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3.

The

The binding specificity of RM130 to Histone H3
acetylated at Lysine 14 (K14ac) is not affected
by the modification of neighboring amino acids.

Western

Western Blot of acid extracts from HeLa cells
untreated (-) or treated with sodium butyrate (+)
or, using RM130 at 0.5 ug/mL.

ChIP

ChIP performed on HeLa cells using H3K14ac
antibody (RM130, 5ug). Real-time PCR was
performed using primers specific to the gene
indicated.

Immunocytochemistry

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H3 (Lys14)
Rabbit mAb RM130 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).


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