Anti-Human IgG4 Rabbit Monoclonal Antibody, Clone RM120

$299.00

Rabbit monoclonal to Human IgG4

SKU: 31-1022-00. Categories: , , . Tags: , .

Product Details
Product NameAnti-Human IgG4 Rabbit Monoclonal Antibody [RM120]
Product DescriptionRabbit monoclonal to Human IgG4
Catalog No.31-1022-00
Clone NameRM120
Product Data SheetAnti-Human IgG4 Rabbit Monoclonal Antibody Data Sheet RM120
SpecificityRM120 reacts to the heavy chain of human IgG4, RM120 does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG3), and shows no cross reactivity to IgM, IgA, IgD, IgE. RM120 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
ImmunogenPeptide corresponding to the hinge region of Human IgG4
ApplicationImmunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Western Blot
Species ReactivityHuman
ConjugateNone
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100ug
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 50ng/well – 200ng/well (for Capture);
0.05ug/mL – 0.2ug/mL (for Detection);
Western Blot (WB): 0.5 ug/mL – 2 ug/mL;
Immunocytochemistry (ICC): 1 ug/mL-10ug/mL;
Immunohistochemistry (IHC): 1 ug/mL-10ug/mL.
Keywords
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Product Images
Detection

Detection of human IgG4 in monkey serum.
Sandwich ELISA using RM120 as the capture
antibody, and biotinylated anti-human light
chains ( κ+ λ) antibody RM129 as the detection
antibody, followed by a HRP conjugated streptavidin.

Sandwich

Sandwich ELISA using RM120 as the capture
antibody, and biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.

Western

Western Blot of human, mouse, rat, and goat
IgG shows RM120 reacts to human IgG4, in
both whole molecule (~150kDa, non-reduced)
and heavy chain (~50kDa, reduced) forms. No
cross reactivity with other isotypes of human IgG,
or mouse, rat, or goat IgG.

A

A titer ELISA using RM120. The plate was coated
with different amounts of human IgG4. A serial dilution
of RM120 was used as the primary antibody, followed
by an alkaline phosphatase conjugated anti-rabbit IgG
as the secondary antibody.

Immunohistochemistry

Immunohistochemical staining of formalin fixed
and paraffin embedded human lymphoid tissue
section using anti-human IgG4 rabbit monoclonal
antibody (Clone RM120).

ELISA

ELISA showing RM120 does not react to monkey
IgG. The plate was coated with Rhesus monkey IgG.
A serial dilution of RM120 and a monkey IgG binding
antibody (positive control) was used as the
detection antibody.


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