Detection of human IgG4 in monkey serum. Sandwich ELISA using RM120 as the capture Western Blot of human, mouse, rat, and goat A titer ELISA using RM120. The plate was coated Immunohistochemical staining of formalin fixed ELISA showing RM120 does not react to monkey Immunohistochemical staining of FFPE Tonsil tissue Image courtesy of Shaomin Hu, MD, PhD,
Product Details
Product Name Anti-Human IgG4 Rabbit Monoclonal Antibody, Clone RM120
Product Description Rabbit monoclonal to Human IgG4
Catalog No. 31-1022-00
Clone Name RM120
Product Data Sheet Anti-Human IgG4 Rabbit Monoclonal Antibody Data Sheet RM120
Specificity RM120 reacts to the heavy chain of human IgG4, RM120 does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG3), and shows no cross reactivity to IgM, IgA, IgD, IgE. RM120 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
Immunogen Peptide corresponding to the hinge region of Human IgG4
Application ELISA
Western Blot
Immunohistochemistry (IHC)
Immunocytochemistry (ICC)
Flow Cytometry (FC)
Species Reactivity Human
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Western Blot (WB): 0.5 µg/mL – 2 µg/mL;
Immunocytochemistry (ICC): 1 µg/mL - 10 µg/mL;
Immunohistochemistry (IHC): 1 µg/mL - 10 µg/mL;
Flow Cytometry (FC): 1 µg/mL - 4 µg/mL.
References for Human IgG4 antibody [RM120] Sulka KB et al. Lyme Disease-Associated IgG4 Autoantibodies Correlate with Synovial Pathology in Antibiotic-Refractory Lyme Arthritis. Arthritis Rheumatol. (2018) 10.1002/art.40566. IHC-P; Human. Read more (PubMed: 29790305)
.
Product Images
Sandwich ELISA using RM120 as the capture
antibody, and biotinylated anti-human light
chains ( κ+ λ) antibody RM129 as the detection
antibody, followed by a HRP conjugated streptavidin.
antibody, and biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.
IgG shows RM120 reacts to human IgG4, in
both whole molecule (~150kDa, non-reduced)
and heavy chain (~50kDa, reduced) forms. No
cross reactivity with other isotypes of human IgG,
or mouse, rat, or goat IgG.
with different amounts of human IgG4. A serial dilution
of RM120 was used as the primary antibody, followed
by an alkaline phosphatase conjugated anti-rabbit IgG
as the secondary antibody.
and paraffin embedded human lymphoid tissue
section using anti-human IgG4 rabbit monoclonal
antibody, Clone RM120.
IgG. The plate was coated with Rhesus monkey IgG.
A serial dilution of RM120 and a monkey IgG binding
antibody (positive control) was used as the
detection antibody.
sections using Anti-Human IgG4 antibody, Clone
RM120, at a 1:50,000 dilution.
Department of Anatomic and Clinical Pathology.
Montefiore Medical Center
Albert Einstein College of Medicine.
Bronx, New York, USA
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