RM172 specifically reacts to Histone H3 Western Blot of acid extracts from HeLa cells Immunocytochemistry of HeLa cells treated with
Product Name Anti-Acetyl-Histone H3 (Lys27) Rabbit Monoclonal Antibody [RM172]
Product Description Rabbit monoclonal to Acetylated Histone H3 Lysine 27 (K27ac), H3K27ac, Histone H3 (K27 acetylation)
Catalog No. 31-1056-00
Clone Name RM172
Product Data Sheet Anti-Acetyl-Histone H3 Lys27 Rabbit Monoclonal Antibody Data Sheet RM172
Specificity RM172 reacts to Histone H3 acetylated at Lysine 27 (K27ac). No cross reactivity with other acetylated Lysines in histone H3.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys27).
Application Western Blot (WB)
Chromatin IP (ChIP)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL - 2 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords References for Acetyl-Histone H3 (Lys27) antibody [RM172] Li Y et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. (2016) 113(50):14402-14407. ChIP; Human.Read more (PubMed: 27911794)
Bulut-Karslioglu A et al. The Transcriptionally Permissive Chromatin State of Embryonic Stem Cells Is Acutely Tuned to Translational Output. Cell Stem Cell. (2018) 10.1016/j.stem.2018.02.004. WB; Human. Read more (PubMed: 29499153)
Lu P et al. Genome encode analyses reveal the basis of convergent evolution of fleshy fruit ripening. Nat Plants. (2018) 10.1038/s41477-018-0249-z. ChIP; Plant. Read more (PubMed: 30250279)
acetylated at Lysine 27 (K27ac). No cross reactivity
with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac),
Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23
(K23ac), Lysine 36 (K36ac), Lysine 56 (K56ac),
Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.
untreated (-) or treated with sodium butyrate (+),
using RM172 at 1 ug/mL, showed a band of
histone H3 acetylated at Lysine 27 in treated HeLa.
sodium butyrate, using Acetyl-Histone H3 (Lys27)
Rabbit mAb RM172 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).
RM172 specifically reacts to Histone H3
Western Blot of acid extracts from HeLa cells
Immunocytochemistry of HeLa cells treated with