Anti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM140; H3K4me1, Histone H3 (monomethyl K4)

$155.00$370.00

Rabbit monoclonal to Monomethylated Histone H3 Lysine 4, H3K4me1, Histone H3 (monomethyl K4)

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Product Details
Product NameAnti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [RM140]
Product DescriptionRabbit monoclonal to Monomethylated Histone H3 Lysine 4, H3K4me1, Histone H3 (monomethyl K4)
Catalog No.31-1046-00
Clone NameRM140
Product Data SheetAnti-Monomethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM140
SpecificityRM140 reacts to Histone H3 monomethylated at Lysine 4 (K4me1). No cross reactivity with dimethylated Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or other methylations in histone H3
ImmunogenA monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys4)
ApplicationWestern Blot (WB)
Chromatin IP (ChIP)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.2 ug/mL – 1 ug/mL;
ChIP: 2 ug/mL- 10 ug/mL;
ICC: 1 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.


Keywords
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Product Images
RM140

RM140 specifically reacts to Histone H3 monomethylated
at Lysine 4 (K4me1). No cross reactivity with dimethylated
Lysine 4 (K4me2), trimethylated Lysine 4 (K4me3), or
other methylations in histone H3.

Western

Western Blot of recombinant histone H3.3 (1) and acid
extracts of HeLa cells (2), using RM140 at 0.5 ug/mL,
showed a band of histone H3 monomethylated at Lysine
4 (K4me1) in HeLa cells.

ChIP

ChIP performed on HeLa cells using H3K4me1 antibody
(RM140, 5ug). Real-time PCR was performed using primers
specific to the gene indicated.

Immunocytochemistry

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Monomethyl-Histone H3
(Lys4) Rabbit mAb RM140 (red). Actin filaments
have been labeled with fluorescein phalloidin (green).


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Size Format

25ug – $155, 100ug – $370

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