Anti-Monomethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM150; H3K9me1, Histone H3 (monomethyl K9)

$155.00$370.00

Rabbit monoclonal to Monomethylated Histone H3 Lysine 9, H3K9me1, Histone H3 (monomethyl K9)

Clear selection

Product Details
Product NameAnti-Monomethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [RM150]
Product DescriptionRabbit monoclonal to Monomethylated Histone H3 Lysine 9, H3K9me1, Histone H3 (monomethyl K9)
Catalog No.31-1064-00
Clone NameRM150
Product Data SheetAnti-Monomethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody Data Sheet RM150
SpecificityRM150 reacts to Histone H3 monomethylated at Lysine 9 (K9me1). No cross reactivity with non-modified Lysine 9, dimethylated Lysine 9 (K9me2), trimethylated Lysine 4 (K9me3), or other methylations in histone H3.
ImmunogenA monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys9).
ApplicationWestern Blot (WB)
Chromatin IP (ChIP)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt.
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.2 ug/mL-1 ug/mL;
ChIP: 2 ug/mL-10 ug/mL
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.



Keywords
.
Product Images
RM150

RM150 specifically reacts to Histone H3 monomethy-
lated at Lysine 9 (K9me1). No cross reactivity with non-
modified Lysine 9, dimethylated Lysine 9 (K9me2),
trimethylated Lysine 4 (K9me3), or other methylations
in histone H3.

Western

Western Blot of recombinant histone H3.3 (1) and
acid extracts of HeLa cells (2), using RM150 at 0.5
ug/mL, showed a band of histone H3 monomethy-
lated at Lysine 9 (K9me1) in HeLa cells.

ChIP performed on HeLa cells using H3K9me1 antibody
(RM150, 5ug). Real-time PCR was performed using
primers specific to the gene indicated.

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Monomethyl-Histone H3
(Lys9) Rabbit mAb RM150 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).


Related Products

Size Format

25ug – $155, 100ug – $370

Related Products