RM149 specifically reacts to Histone H3 acetylated Western Blot of acid extracts from HeLa cells Immunocytochemistry of HeLa cells treated with
Product Details
Product Name Anti-Acetyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [RM149]
Product Description Rabbit monoclonal to Acetylated Histone H3 Lysine 4 (K4ac), H3K4ac, Histone H3 (K4 acetylation)
Catalog No. 31-1063-00
Clone Name RM149
Product Data Sheet Anti-Acetyl-Histone H3 Lys4 Rabbit Monoclonal Antibody Data Sheet RM149
Specificity RM149 reacts to Histone H3 acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H3.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys4).
Application Chromatin IP (ChIP)
Western Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ChIP: 1µg-5 µg;
Western Blot: 0.25 ug/mL - 1 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.05 ug/mL – 0.5 ug/mL.
Keywords
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References Mondal B et al The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth. Elife. (2020), 10.7554/eLife.57519. WB; Human. Read more (PubMed: 32297854)
Product Images
at Lysine 4 (K4ac). No cross reactivity with non-
modified Lysine 4, acetylated Lysine 9 (K9ac), Lysine
14 (K14ac), Lysine 18 (K18ac),Lysine 23 (K23ac),
Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56
(K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in
histone H3.
untreated (-) or treated with sodium butyrate (+),
using RM149 at 0.5 ug/mL, showed a band of
histone H3 acetylated at Lysine 4 in treated HeLa.
sodium butyrate, using Acetyl-Histone H3 (Lys4)
Rabbit mAb RM149 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).
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| Size Format | 25ug – $194, 100ug – $432 |
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Reference
Mondal B et al The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth. Elife. (2020), 10.7554/eLife.57519. WB; Human. Read more (PubMed: 32297854)