Anti-Acetyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM149; H3K4ac, Histone H3 (K4 acetylation)

Product Details
Product Name	Anti-Acetyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [RM149]
Product Description	Rabbit monoclonal to Acetylated Histone H3 Lysine 4 (K4ac), H3K4ac, Histone H3 (K4 acetylation)
Catalog No.	31-1063-00
Clone Name	RM149
Product Data Sheet	Anti-Acetyl-Histone H3 Lys4 Rabbit Monoclonal Antibody Data Sheet RM149
Specificity	RM149 reacts to Histone H3 acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H3.
Immunogen	An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys4).
Application	Western Blot (WB) Immunocytochemistry (ICC) ELISA Multiplex
Species Reactivity	Human Vertebrates
Conjugate	None
Intended Use	Research Use Only
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Properties
Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer	50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size	100 ug
Concentration	1.0 mg/mL
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Usage	Western Blot: 0.25 ug/mL - 1 ug/mL; ICC: 0.5 ug/mL - 2 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.05 ug/mL – 0.5 ug/mL.
Keywords
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Product Images
	RM149 specifically reacts to Histone H3 acetylatedat Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4, acetylated Lysine 9 (K9ac), Lysine14 (K14ac), Lysine 18 (K18ac),Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), lysine 79 (K79ac), or Lysine 122 (K122) inhistone H3.
	Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM149 at 0.5 ug/mL, showed a band of histone H3 acetylated at Lysine 4 in treated HeLa.
	Immunocytochemistry of HeLa cells treated withsodium butyrate, using Acetyl-Histone H3 (Lys4) Rabbit mAb RM149 (red). Actin filaments havebeen labeled with fluorescein phalloidin (green).