Anti-Acetyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM149; H3K4ac, Histone H3 (K4 acetylation)


Rabbit monoclonal to Acetylated Histone H3 Lysine 4 (K4ac), H3K4ac, Histone H3 (K4 acetylation)

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Product Details
Product NameAnti-Acetyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [RM149]
Product DescriptionRabbit monoclonal to Acetylated Histone H3 Lysine 4 (K4ac), H3K4ac, Histone H3 (K4 acetylation)
Catalog No.31-1063-00
Clone NameRM149
Product Data SheetAnti-Acetyl-Histone H3 Lys4 Rabbit Monoclonal Antibody Data Sheet RM149
SpecificityRM149 reacts to Histone H3 acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H3.
ImmunogenAn acetyl-peptide corresponding to Acetyl-Histone H3 (Lys4).
ApplicationWestern Blot (WB)
Immunocytochemistry (ICC)
Species ReactivityHuman
Intended UseResearch Use Only
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.25 ug/mL - 1 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.05 ug/mL – 0.5 ug/mL.

Product Images

RM149 specifically reacts to Histone H3 acetylated
at Lysine 4 (K4ac). No cross reactivity with non-
modified Lysine 4, acetylated Lysine 9 (K9ac), Lysine
14 (K14ac), Lysine 18 (K18ac),Lysine 23 (K23ac),
Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56
(K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in
histone H3.


Western Blot of acid extracts from HeLa cells
untreated (-) or treated with sodium butyrate (+),
using RM149 at 0.5 ug/mL, showed a band of
histone H3 acetylated at Lysine 4 in treated HeLa.


Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H3 (Lys4)
Rabbit mAb RM149 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).

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25ug – $155, 100ug – $360

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