RM149 specifically reacts to Histone H3 acetylated Western Blot of acid extracts from HeLa cells Immunocytochemistry of HeLa cells treated with
Product Name Anti-Acetyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [RM149]
Product Description Rabbit monoclonal to Acetylated Histone H3 Lysine 4 (K4ac), H3K4ac, Histone H3 (K4 acetylation)
Catalog No. 31-1063-00
Clone Name RM149
Product Data Sheet Anti-Acetyl-Histone H3 Lys4 Rabbit Monoclonal Antibody Data Sheet RM149
Specificity RM149 reacts to Histone H3 acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H3.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys4).
Application Chromatin IP (ChIP)
Western Blot (WB)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ChIP: 1µg-5 µg;
Western Blot: 0.25 ug/mL - 1 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.05 ug/mL – 0.5 ug/mL.
Keywords . References Mondal B et al The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth. Elife. (2020), 10.7554/eLife.57519. WB; Human. Read more (PubMed: 32297854)
at Lysine 4 (K4ac). No cross reactivity with non-
modified Lysine 4, acetylated Lysine 9 (K9ac), Lysine
14 (K14ac), Lysine 18 (K18ac),Lysine 23 (K23ac),
Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56
(K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in
untreated (-) or treated with sodium butyrate (+),
using RM149 at 0.5 ug/mL, showed a band of
histone H3 acetylated at Lysine 4 in treated HeLa.
sodium butyrate, using Acetyl-Histone H3 (Lys4)
Rabbit mAb RM149 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).
RM149 specifically reacts to Histone H3 acetylated
Western Blot of acid extracts from HeLa cells
Immunocytochemistry of HeLa cells treated with