Anti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody, Clone RM155; H3K36me3, Histone H3 (trimethyl K36)

$155.00$375.00

Rabbit monoclonal to Trimethylated Histone H3 Lysine 36, H3K36me3, Histone H3 (trimethyl K36)

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Product Details
Product NameAnti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody [RM155]
Product DescriptionRabbit monoclonal to Trimethylated Histone H3 Lysine 36, H3K36me3, Histone H3 (trimethyl K36)
Catalog No.31-1051-00
Clone NameRM155
Product Data SheetAnti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody Data Sheet RM155
SpecificityRM155 reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with monomethylated Lysine 36 (K36me1), dimethylated Lysine 36 (K36me2), or other methylations in histone H3.
ImmunogenA trimethyl-peptide corresponding to trimethyl-Histone H3 (Lys36).
ApplicationWestern Blot (WB)
Chromatin IP (ChIP)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL – 2 ug/mL;
ChIP: 2 ug/mL- 10 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.

Keywords
References for Trimethyl-Histone H3 (Lys36) antibody [RM155]References for Acetyl-Histone H3 (Lys23) antibody [RM169]
Product Images
RM155

RM155 specifically reacts to Histone H3 trimethylated
at Lysine 36 (K36me3). No cross reactivity with non-
modified Lysine 36 (K36 Ctrl), monomethylated Lysine
36 (K36me1) or dimethylated Lysine 36 (K36me2), or
other methylations in Histone H3.

Western

Western Blot of recombinant histone H3.3 (1)
and acid extracts of HeLa cells (2), using RM155 at
2.0 ug/mL, showed a band of histone H3
trimethylated at Lysine 36 (K36me3) in HeLa cells.

ChIP

ChIP performed on HeLa cells using H3K36me3 antibody
(RM155, 5ug). Real-time PCR was performed using primers
specific to the gene indicated.


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Size Format

25ug – $155, 100ug – $375

References

Cheung P et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell. (2018) 10.1016/j.cell.2018.03.079. Mass Cytometry; Human.Read more (PubMed: 29706550)