Anti-Acetyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody, Clone RM154; H3K36ac, Histone H3 (K36 acetylation)

$155.00

Rabbit monoclonal to Acetylated Histone H3 Lysine 36 (K36ac), H3K36ac, Histone H3 (K36 acetylation)

SKU: 31-1050-00.

Product Details
Product NameAnti-Acetyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody [RM154]
Product DescriptionRabbit monoclonal to Acetylated Histone H3 Lysine 36 (K36ac), H3K36ac, Histone H3 (K36 acetylation)
Catalog No.31-1050-00
Clone NameRM154
Product Data SheetAnti-Acetyl-Histone H3 Lys36 Rabbit Monoclonal Antibody Data Sheet RM154
SpecificityRM154 reacts to Histone H3 acetylated at Lysine 36 (K36ac), and is not affected by the phosphorylation of neighboring Ser31. No cross reactivity with other acetylated Lysines in histone H3.
ImmunogenAn acetyl-peptide corresponding to Acetyl-Histone H3 (Lys36).
ApplicationWestern Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL - 2 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
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Product Images
RM154

RM154 specifically reacts to Histone H3 acetylated
at Lysine 36 (K36ac), and is not affected by the
phosphorylation of neighboring Ser31. No cross
reactivity with acetylated Lysine 4 (K4ac), Lysine 9
(K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac),
Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 56
(K56ac), lysine 79 (K79ac), or Lysine 122 (K122)
in histone H3.

Western

Western Blot of acid extracts from HeLa cells
untreated (-) or treated with sodium butyrate (+),
using RM154 at 1.0 ug/mL, showed a band of
histone H3 acetylated at Lysine 36 in treated
HeLa.

Immunocytochemistry

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H3 (Lys36)
Rabbit mAb RM154 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).


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