Immunohistochemistry of mouse liver and kidney ELISA of PEGylated BSA using Biotinylated anti-PEG Western blot of BSA and PEGylated BSA (mPEG 5 kDa)
Product Details
Product Name Biotin Anti-PEG (methoxy group) Rabbit Monoclonal Antibody, Clone RM105
Description Biotinylated Rabbit monoclonal to Polyethylene Glycol
Catalog No. 31-1008-02
Clone Name RM105
Product Data Sheet Biotin Anti-mPEG Rabbit Monoclonal Antibody Data Sheet RM105
Specificity RM105 reacts to the methoxy group of Polyethylene glycol (PEG). It is specific for methoxypolyethylene glycol.
Immunogen KLH-PEG with terminal methoxy group
Application ELISA
Immunohistochemistry (IHC)
Western Blot (WB)
Species Reactivity All Species
Conjugate Biotin
Intended Use Research Use Only
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Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 50 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.02 µg/mL - 0.5 µg/mL;
Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL;
Western Blot (WB) : 0.1 µg/mL - 1 µg/mL.
Keywords
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Product Images
using 0.5 µg/mL of RevMAb anti-PEG Clone RM105
biotin conjugate, followed by an HRP conjugated
streptavidin. The mouse was injected with PEG-BSA
or BSA 3 hours before sampling.
rabbit monoclonal antibody, Clone RM105. The ELISA
plate was coated with 50 µL/well of BSA-PEG at 1 µg/mL,
0.33 µg/mL, 0.11 µg/mL, and 0.04 µg/mL. Different
concentrations of Biotinylated anti-PEG mAb, Clone
RM105, was used as the primary antibody, followed
by an AP conjugated Streptavidin.
using 0.1 µg/mL of anti-PEG RevMAb Clone RM105.
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