Anti-Histone H3 K36M Rabbit Monoclonal Antibody, Clone RM193; Histone H3, K36M Mutant
$362.00 – $881.00
Rabbit monoclonal to Histone H3 K36M;
Histone H3, K36M Mutant
100 µL Small Size, 400 µL Large Size, 50 µL Biotin Conjugate
Request Quote for Bulk Sizes
Western Blot analysis of cell lysates prepared from Immunohistochemical staining of formalin fixed and Immunohistochemical staining of formalin fixed Image courtesy of Adrienne Flanagan, Department
Product Details
Product Name Anti-Histone H3 K36M Rabbit Monoclonal Antibody, Clone RM193
Product Description Rabbit monoclonal to Histone H3 K36M; Histone H3, K36M Mutant
Catalog No. 31-1085-00-S;
31-1085-00-L;
31-1085-02
Clone Name RM193
Product Data Sheet Anti-Histone H3 K36M Rabbit Monoclonal Antibody Data Sheet RM193
Biotinylated Anti-Histone H3 K36M Rabbit Monoclonal Antibody Data Sheet RM193
Specificity RM193 reacts to the Histone H3 K36M mutant. No cross reactivity with wild type Histone H3.
Immunogen A peptide corresponding to Histone H3 K36M mutant
Application Western Blot
ELISA
Immunohistochemistry
Chromatin IP
Species Reactivity All
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 µL;
400 µL;
Biotinylated 50 µL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage WB: 1:200-1:1000;
ELISA: 1:250-1:2500;
IHC: 1:100-1:500.
Keywords H3F3A ; H3F3B ; K36M; bone tumour; chondroblastoma
References for Histone H3 K36M Mutant antibody [RM193] Papke D J et al. Clinicopathologic characterization of malignant chondroblastoma: a neoplasm with locally aggressive behavior and metastatic potential that closely mimics chondroblastoma-like osteosarcoma. Mod Pathol. (2020), 10.1038/s41379-020-0604-2. IHC; Human. Read more (PubMed: 32601382)
Brumbaugh J et al. Inducible histone K-to-M mutations are dynamic tools to probe the physiological role of site-specific histone methylation in vitro and in vivo. Nat Cell Biol. (2019), 10.1038/s41556-019-0403-5. WB; Human. Read more (PubMed: 31659274)
Lu C et al. Histone H3K36M mutation and trimethylation patterns in chondroblastoma. Histopathology. (2019), 10.1111/his.13725. IHC; Human. Read more (PubMed: 30098026)
Amary F et al. Synovial chondromatosis and soft tissue chondroma: extraosseous cartilaginous tumor defined by FN1 gene rearrangement. Mod Pathol. (2019), 10.1038/s41379-019-0315-8. IHC; Human. Read more (PubMed: 31273315)
Fang D et al. Probing the Function of Oncohistones Using Mutant Transgenes and Knock-In Mutations. Methods Mol Biol. (2018), 10.1007/978-1-4939-8663-7_19. IHC-P; Human. Read more (PubMed: 30073537)
Frye J et al. H3F3 K36M Immunohistochemistry is a Useful Diagnostic Marker for Chondroblastoma in Small Biopsy Specimens. Mod Pathol. (2018), USCAP 2018 Abstracts-Bone & Soft Tissue Pathology (45-120). IHC-P; Human. Read more (USCAP 2018 Abstracts: 63: PubMed: 29551782)
Amary MF et al. The H3F3 K36M mutant antibody is a sensitive and specific marker for the diagnosis of chondroblastoma. Histopathology (2016) 10.1111/his.12945. IHC-P; Human. Read more (PubMed: 26844533)
Fang D et al. The histone H3.3K36M mutation reprograms the epigenome of chondroblastomas. Science (2016) 10.1126/science.aae0065. ChIP; Human. Read more (PubMed: 27229140)
Jundt G et al. Chondroblastoma. Der Pathologe (2018) 10.1007/s00292-017-0397-3. IHC-P; Human Read more (PubMed: 29209799)
Schaefer IM et al. Immunohistochemistry for histone H3G34W and H3K36M is highly specific for giant cell tumor of bone and chondroblastoma, respectively, in FNA and core needle biopsy. Cancer Cytopathol. (2018) 10.1002/cncy.22000. IHC-P; Human. Read more (PubMed: 29757500)
.
Product Images
293T transfected with a DNA construct encoding
wild type or K36M mutant proteins of Histone H3.1
and H3.3, using anti-Histone H3 K36M, Clone RM193.
paraffin embedded 293T cells transfected with a DNA
construct encoding Histone H3 K36M, stained with
anti-Histone H3 K36M, Clone RM193.
paraffin embedded (FFPE) Chondroblastoma tumor
cell tissue sections using RM193 Anti-Histone H3
K36M mAb at a 1:400 dilution.
of Histopathology, RONH, Stanmore, Middlesex, UK
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Anti-Histone H3 K36M Rabbit Monoclonal Antibody, Clone RM193; Histone H3, K36M Mutant
| Conjugation | Unconjugated, Biotin Conjugate |
|---|---|
| Size format | 50 µL, 100 µL, 400 µL |
References
Papke D J et al. Clinicopathologic characterization of malignant chondroblastoma: a neoplasm with locally aggressive behavior and metastatic potential that closely mimics chondroblastoma-like osteosarcoma. Mod Pathol. (2020), 10.1038/s41379-020-0604-2. IHC; Human. Read more (PubMed: 32601382)
Brumbaugh J et al. Inducible histone K-to-M mutations are dynamic tools to probe the physiological role of site-specific histone methylation in vitro and in vivo. Nat Cell Biol. (2019), 10.1038/s41556-019-0403-5. WB; Human. Read more (PubMed: 31659274)
Lu C et al. Histone H3K36M mutation and trimethylation patterns in chondroblastoma. Histopathology. (2019), 10.1111/his.13725. IHC; Human. Read more (PubMed: 30098026)
Amary F et al. Synovial chondromatosis and soft tissue chondroma: extraosseous cartilaginous tumor defined by FN1 gene rearrangement. Mod Pathol. (2019), 10.1038/s41379-019-0315-8. IHC; Human. Read more (PubMed: 31273315)
Fang D et al. Probing the Function of Oncohistones Using Mutant Transgenes and Knock-In Mutations. Methods Mol Biol. (2018), 10.1007/978-1-4939-8663-7_19. IHC-P; Human. Read more (PubMed: 30073537)
Rehkamper J et al. Diagnostic tools in the differential diagnosis of giant cell-rich lesions of bone at biopsy. Oncotarget. (2018), 10.18632/oncotarget.25725. IHC-P; Human. Read more (PubMed: PMC6059026)
Frye J et al. H3F3 K36M Immunohistochemistry is a Useful Diagnostic Marker for Chondroblastoma in Small Biopsy Specimens. Mod Pathol. (2018), USCAP 2018 Abstracts-Bone & Soft Tissue Pathology (45-120). IHC-P; Human. Read more (USCAP 2018 Abstracts: 63: PubMed: 29551782)
Schaefer IM et al. Immunohistochemistry for histone H3G34W and H3K36M is highly specific for giant cell tumor of bone and chondroblastoma, respectively, in FNA and core needle biopsy. Cancer Cytopathol. (2018) 10.1002/cncy.22000. IHC-P; Human. Read more (PubMed: 29757500)
Amary MF et al. The H3F3 K36M mutant antibody is a sensitive and specific marker for the diagnosis of chondroblastoma. Histopathology (2016) 10.1111/his.12945. IHC-P; Human. Read more (PubMed: 26844533)
Fang D et al. The histone H3.3K36M mutation reprograms the epigenome of chondroblastomas. Science (2016) 10.1126/science.aae0065. ChIP; Human. Read more (PubMed: 27229140)
Jundt G et al. Chondroblastoma. Der Pathologe (2018) 10.1007/s00292-017-0397-3. IHC-P; Human Read more (PubMed: 29209799)