Anti-Acetyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody, Clone RM166; H3K18ac, Histone H3 (K18 acetylation)

$155.00$370.00

Rabbit monoclonal to Acetylated Histone H3 Lysine 18 (K18ac), H3K18ac, Histone H3 (K18 acetylation)

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Product Details
Product NameAnti-Acetyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody [RM166]
Product DescriptionRabbit monoclonal to Acetylated Histone H3 Lysine 18 (K18ac), H3K18ac, Histone H3 (K18 acetylation)
Catalog No.31-1055-00
Clone NameRM166
Product Data SheetAnti-Acetyl-Histone H3 Lys18 Rabbit Monoclonal Antibody Data Sheet RM166
SpecificityRM166 reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with other acetylated Lysines in histone H3.
ImmunogenAn acetyl-peptide corresponding to Acetyl-Histone H3 (Lys18).
ApplicationWestern Blot (WB)
Chromatin IP (ChIP)
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL - 2 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ChIP: 2 ug/mL-10 ug/mL;
IHC: 1 ug/mL-10 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.

Keywords
References for Acetyl-Histone H3 (Lys18) antibody [RM166]Li Y et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. (2016) 113(50):14402-14407. ChIP; Human.Read more (PubMed: 27911794)

Cheung P et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell. (2018) 10.1016/j.cell.2018.03.079. Mass Cytometry; Human.Read more (PubMed: 29706550)

Product Images
RM166

RM166 specifically reacts to Histone H3
acetylated at Lysine 18 (K18ac). No cross reactivity
with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac),
Lysine 14 (K14ac), Lysine 23 (K23ac), Lysine 27
(K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac),
Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.

Western

Western Blot of acid extracts from HeLa cells
untreated (-) or treated with sodium butyrate (+),
using RM166 at 0.5 ug/mL, showed a band of
histone H3 acetylated at Lysine 18 in treated HeLa.

Sandwich

Sandwich ELISA against acetylated histone H3
at Lys 18 using HeLa whole cell lysate, treated or
untreated with Sodium Butyrate. Using anti-H3CT
(RM188, 1 ug/mL) as the capture antibody and
biotinylated anti-H3K18C (RM166, 2 ug/mL) as the
detection antibody.

ChIP

ChIP performed on HeLa cells with or without
Sodium Butyrate treatment, using H3K18Ac
antibody (RM166, 5ug). Real-time PCR was
performed using primers specific to the gene
indicated.

Immunocytochemistry

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H3 (Lys18)
Rabbit mAb RM166(red). Actin filaments have
been labeled with fluorescein phalloidin (green).

Immunohistochemistry

Immunohistochemistry staining of HepG2 cells using
Anti-Acetyl-Histone H3 (Lys18) antibody, RM166.


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Size Format

25ug – $155, 100ug – $370

References

Li Y et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. (2016) 113(50):14402-14407. ChIP; Human.Read more (PubMed: 27911794)

Cheung P et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell. (2018) 10.1016/j.cell.2018.03.079. Mass Cytometry; Human.Read more (PubMed: 29706550)