RM166 specifically reacts to Histone H3 Western Blot of acid extracts from HeLa cells Sandwich ELISA against acetylated histone H3 ChIP performed on HeLa cells with or without Immunocytochemistry of HeLa cells treated with Immunohistochemistry staining of HepG2 cells using
Product Name Anti-Acetyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody [RM166]
Product Description Rabbit monoclonal to Acetylated Histone H3 Lysine 18 (K18ac), H3K18ac, Histone H3 (K18 acetylation)
Catalog No. 31-1055-00
Clone Name RM166
Product Data Sheet Anti-Acetyl-Histone H3 Lys18 Rabbit Monoclonal Antibody Data Sheet RM166
Specificity RM166 reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with other acetylated Lysines in histone H3.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys18).
Application Western Blot (WB)
Chromatin IP (ChIP)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL - 2 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ChIP: 2 ug/mL-10 ug/mL;
IHC: 1 ug/mL-10 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords References for Acetyl-Histone H3 (Lys18) antibody [RM166] Li Y et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. (2016) 113(50):14402-14407. ChIP; Human.Read more (PubMed: 27911794)
Cheung P et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell. (2018) 10.1016/j.cell.2018.03.079. Mass Cytometry; Human.Read more (PubMed: 29706550)
acetylated at Lysine 18 (K18ac). No cross reactivity
with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac),
Lysine 14 (K14ac), Lysine 23 (K23ac), Lysine 27
(K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac),
Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.
untreated (-) or treated with sodium butyrate (+),
using RM166 at 0.5 ug/mL, showed a band of
histone H3 acetylated at Lysine 18 in treated HeLa.
at Lys 18 using HeLa whole cell lysate, treated or
untreated with Sodium Butyrate. Using anti-H3CT
(RM188, 1 ug/mL) as the capture antibody and
biotinylated anti-H3K18C (RM166, 2 ug/mL) as the
Sodium Butyrate treatment, using H3K18Ac
antibody (RM166, 5ug). Real-time PCR was
performed using primers specific to the gene
sodium butyrate, using Acetyl-Histone H3 (Lys18)
Rabbit mAb RM166(red). Actin filaments have
been labeled with fluorescein phalloidin (green).
Anti-Acetyl-Histone H3 (Lys18) antibody, RM166.
RM166 specifically reacts to Histone H3
Western Blot of acid extracts from HeLa cells
Sandwich ELISA against acetylated histone H3
ChIP performed on HeLa cells with or without
Immunocytochemistry of HeLa cells treated with
Immunohistochemistry staining of HepG2 cells using