RM154 specifically reacts to Histone H3 acetylated Western Blot of acid extracts from HeLa cells Immunocytochemistry of HeLa cells treated with
Product Name Anti-Acetyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody [RM154]
Product Description Rabbit monoclonal to Acetylated Histone H3 Lysine 36 (K36ac), H3K36ac, Histone H3 (K36 acetylation)
Catalog No. 31-1050-00
Clone Name RM154
Product Data Sheet Anti-Acetyl-Histone H3 Lys36 Rabbit Monoclonal Antibody Data Sheet RM154
Specificity RM154 reacts to Histone H3 acetylated at Lysine 36 (K36ac), and is not affected by the phosphorylation of neighboring Ser31. No cross reactivity with other acetylated Lysines in histone H3.
Immunogen An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys36).
Application Western Blot (WB)
Species Reactivity Human
Intended Use Research Use Only
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.5 ug/mL - 2 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords . Product Images
at Lysine 36 (K36ac), and is not affected by the
phosphorylation of neighboring Ser31. No cross
reactivity with acetylated Lysine 4 (K4ac), Lysine 9
(K9ac), Lysine 14 (K14ac), Lysine 18 (K18ac),
Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 56
(K56ac), lysine 79 (K79ac), or Lysine 122 (K122)
in histone H3.
untreated (-) or treated with sodium butyrate (+),
using RM154 at 1.0 ug/mL, showed a band of
histone H3 acetylated at Lysine 36 in treated
sodium butyrate, using Acetyl-Histone H3 (Lys36)
Rabbit mAb RM154 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).
RM154 specifically reacts to Histone H3 acetylated
Western Blot of acid extracts from HeLa cells
Immunocytochemistry of HeLa cells treated with