Anti-Acetyl-Histone H3 (Lys56) Rabbit Monoclonal Antibody, Clone RM179; H3K56ac, Histone H3 (K56 acetylation)

$155.00$365.00

Rabbit monoclonal to Acetylated Histone H3 Lysine 56 (K56ac), H3K56ac, Histone H3 (K56 acetylation)

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Product Details
Product NameAnti-Acetyl-Histone H3 (Lys56) Rabbit Monoclonal Antibody [RM179]
Product DescriptionRabbit monoclonal to Acetylated Histone H3 Lysine 56 (K56ac), H3K56ac, Histone H3 (K56 acetylation)
Catalog No.31-1078-00
Clone NameRM179
Product Data SheetAnti-Acetyl-Histone H3 Lys56 Rabbit Monoclonal Antibody Data Sheet RM179
SpecificityRM179 reacts to Histone H3 acetylated at Lysine 56 (K56ac). No cross reactivity with other acetylated Lysines in histone H3.
ImmunogenAn acetyl-peptide corresponding to Acetyl-Histone H3 (Lys56).
ApplicationWestern Blot (WB)
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)
Chromatin IP (ChIP)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 1 ug/mL - 2 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
IHC: 1 ug/mL – 10 ug/mL;
ELISA: 0.5 ug/mL - 1 ug/mL;
Multiplex: 0.5 ug/mL – 2 ug/mL.

Keywords
References for Acetyl-Histone H3 (Lys56) antibody [RM179]Li Y et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. (2016) 113(50):14402-14407. ChIP; Human.Read more (PubMed: 27911794)
Product Images
Western

Western Blot of (1) acid extracts of HeLa cells treated with
sodium butyrate and (2) recombinant histone H3.3, using
RM179 at 1 µg/mL, showed a band of histone H3 acetylated
at Lysine 56 in treated HeLa.

RM179

RM179 specifically reacts to Histone H3 acetylated at Lysine 56
(K56ac). No cross reactivity with unmodified Lysine 56 (K56 ctrl),
acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac),
Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36
(K36ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.

Immunocytochemistry

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H3 (Lys56)
Rabbit mAb RM179 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).

Immunohistochemistry

Immunohistochemistry staining of HepG2 cells using
Anti-Acetyl-Histone H3 (Lys56) antibody, RM179.


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Size Format

25ug – $155, 100ug – $365

References

Li Y et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. (2016) 113(50):14402-14407. ChIP; Human.Read more (PubMed: 27911794)