Anti-Acetyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody, Clone RM156; H3K79ac, Histone H3 (K79 acetylation)

$155.00

Rabbit monoclonal to Acetylated Histone H3 Lysine 79 (K79ac), H3K79ac, Histone H3 (K79 acetylation)

SKU: 31-1052-00.

Product Details
Product NameAnti-Acetyl-Histone H3 (Lys79) Rabbit Monoclonal Antibody [RM156]
Product DescriptionRabbit monoclonal to Acetylated Histone H3 Lysine 79 (K79ac), H3K79ac, Histone H3 (K79 acetylation)
Catalog No.31-1052-00
Clone NameRM156
Product Data SheetAnti-Acetyl-Histone H3 Lys79 Rabbit Monoclonal Antibody Data Sheet RM156
SpecificityRM156 reacts to Histone H3 acetylated at Lysine 79 (K79ac). No cross reactivity with other acetylated Lysines in Histone H3.
ImmunogenAn acetyl-peptide corresponding to Acetyl-Histone H3 (Lys79).
ApplicationWestern Blot (WB)
Chromatin IP (ChIP)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL - 2 ug/mL;
ICC: 0.5 ug/mL - 2 ug/mL;
ChIP: 2 ug/mL- 10 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.05 ug/mL – 0.2 ug/mL.

Keywords
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Product Images
RM156

RM156 specifically reacts to Histone H3 acetylated
at Lysine 79 (K79ac). No cross reactivity with acetyl-
ated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14
(K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac),
Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56
(K56ac), or Lysine 122 (K122) in Histone H3.

Western

Western Blot of acid extracts from HeLa cells
untreated (-) or treated with sodium butyrate (+),
using RM156 at 1.0 ug/mL, showed a band of
histone H3 acetylated at Lysine 79 in treated
HeLa.

Sandwich

Sandwich ELISA against acetylated histone H3
at Lys 79 using HeLa whole cell lysate, treated or
untreated with Sodium Butyrate. Using anti-H3K79ac
(RM156, 5 ug/mL) as the capture antibody and
biotinylated anti-H3CT (RM188, 1 ug/mL) as the
detection antibody.

ChIP

ChIP performed on HeLa cells with or without
Sodium Butyrate treatment, using H3K79Ac
antibody (RM156, 5ug). Real-time PCR was
performed using primers specific to the gene
indicated.

Immunocytochemistry

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Acetyl-Histone H3 (Lys79)
Rabbit mAb RM156 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).


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