Anti-Acetyl-Histone H3 (Lys23) Rabbit Monoclonal Antibody, Clone RM169; H3K23ac, Histone H3 (K23 acetylation)

$155.00$365.00

Rabbit monoclonal to Acetylated Histone H3 Lysine 23 (K23ac), H3K23ac, Histone H3 (K23 acetylation)

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Product Details
Product NameAnti-Acetyl-Histone H3 (Lys523) Rabbit Monoclonal Antibody [RM169]
Product DescriptionRabbit monoclonal to Acetylated Histone H3 Lysine 23 (K23ac), H3K23ac, Histone H3 (K23 acetylation)
Catalog No.31-1087-00
Clone NameRM169
Product Data SheetAnti-Acetyl-Histone H3 Lys23 Rabbit Monoclonal Antibody Data Sheet RM169
SpecificityRM169 reacts to Histone H3 acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in histone H3.
ImmunogenAn acetyl-peptide corresponding to Acetyl-Histone H3 (Lys23).
ApplicationWestern Blot (WB)
ELISA
Multiplex
Immunocytochemistry (ICC)
Species ReactivityHuman
Vertebrates
ConjugateNone
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size100 ug
Concentration 1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageWestern Blot: 0.5 ug/mL - 2 ug/mL;
ELISA: 0.5 ug/mL - 1 ug/mL;
Multiplex: 0.2 ug/mL – 1 ug/mL;
Immunocytochemistry: 1 ug/mL- 2 ug/mL.

Keywords
References for Acetyl-Histone H3 (Lys23) antibody [RM169]Cheung P et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell. (2018) 10.1016/j.cell.2018.03.079. Mass Cytometry; Human.Read more (PubMed: 29706550)
Product Images
Western

Western Blot of acid extracts of HeLa cells non-treated
(1) or treated with sodium butyrate (2), and recombinant
histone H3.3 (3), using RM169 at 1 ug/mL, showed a
band of histone H3 acetylated at Lysine 23 in treated HeLa.

RM169

RM169 specifically reacts to Histone H3 acetylated at Lysine 23
(K23ac). No cross reactivity with unmodified Lysine 23 (K23 ctrl),
acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 14 (K14ac),
Lysine 18 (K18ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56
(K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.

Immunocytochemical

Immunocytochemical staining of HeLa cells treated
with sodium butyrate, using anti-Acetyl-Histone
H2A.Z (Lys4) Rabbit Monoclonal Antibody (clone
RM221) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).


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Size Format

25ug – $155, 100ug – $365

References

Cheung P et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell. (2018) 10.1016/j.cell.2018.03.079. Mass Cytometry; Human.Read more (PubMed: 29706550)