RM217 specifically reacts to human IgG4. No cross Sandwich ELISA using RM217 as the capture Sandwich ELISA using RM217 as the capture A titer ELISA using RM217. The plate was
Product Details
Product Name Anti-Human IgG4 Fc Rabbit Monoclonal Antibody, Clone RM217
Product Description Rabbit monoclonal to Human IgG4 Fc
Catalog No. 31-1100-00
Clone Name RM217
Product Data Sheet Anti-Human IgG4 Fc Rabbit Monoclonal Antibody Data Sheet RM217
Specificity RM217 reacts to the Fc region of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Immunogen Peptide corresponding to the Fc region of Human IgG4
Application ELISA
Species Reactivity Human
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100 µg
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection).
Keywords
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Product Images
reactivity with human IgG1, IgG2, IgG3.
antibody, and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.
antibody, and Biotinylated anti-human light chains
( κ+ λ) antibody RM129 as the detection antibody,
followed by an AP conjugated streptavidin.
coated with different amounts of human IgG4.
A serial dilution of RM217 was used as the
primary antibody. An alkaline phosphatase
conjugated anti-rabbit IgG as the secondary
antibody.
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